​Integral to fraternal life and the spiritual formation of seminarians are the many and varied opportunities for personal and community prayer and worship at Theological College. Seminarians meet for morning and evening prayer and participate in the celebration of the Eucharist in the seminary chapel throughout the week. Private prayer and Adoration are also nurtured. The seminary hosts exposition of the Blessed Sacrament in the main chapel two days per week, and also one evening per week in Caldwell Hall on the Catholic University campus.

The many liturgies and other formational activities occurring through the year, such as the regular celebration of individual feast days, Sulpician meditations, communal rosary, days of recollection, and evenings and weekends of silence, help seminarians develop solid habits of prayer that impart a spiritual vitality, build virtue, and support collegial awareness. The maturation of this solid prayer life will sustain them during their time in seminary and in their ministry as priests.